Rapid and convenient electrochemical hemoglobin detection in mouse feces employing a DNA aptamer to evaluate the severity of colitis in a mouse model

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요약

Anal. Methods, 2026, Advance ArticleDOI: 10.1039/D6AY00278A, CommunicationMizuki Nakashima, Daimei Miura, Erika Komiya, Nanami Watanabe, Kaori Tsukakoshi, Tsutomu Omatsu, Wakako Tsugawa, Koji Sode, Kazunori Ikebukuro, Toshinori Yoshida, Ryutaro AsanoMouse hem…

본문

Rapid and convenient electrochemical hemoglobin detection in mouse feces employing a DNA aptamer to evaluate the severity of colitis in a mouse model

* Corresponding authors

a Department of Biotechnology and Life Science, Graduate School of Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo, Japan
E-mail: [email protected]

b Institute of Global Innovation Research, Tokyo University of Agriculture and Technology, 3-8-1 Harumi-cho, Fuchu, Tokyo, Japan

c Department of Veterinary Medicine, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu, Tokyo 183-8509, Japan

d Department of Chemistry, Faculty of Science Division I, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku, Tokyo 162-8601, Japan

e Center for Infectious Disease Epidemiology and Prevention Research, Faculty of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu, Tokyo 183-8509, Japan

f Lampe Joint Department of Biomedical Engineering, University of North Carolina at Chapel Hill, North Carolina State University, Chapel Hill, NC 27599, USA

Abstract

We established a rapid and convenient electrochemical detection system for quantifying mouse fecal hemoglobin to evaluate colitis severity. A human myoglobin-binding aptamer was cross-reactive with mouse hemoglobin, and coupled with redox enzymes, enabled sensitive detection in feces (12.5–200 nM) within 20 min. This highlights the potential of cross-species hemoglobin detection.